Gram staining: to differentiate Gram-positive from Gram-negative bacteria

Gram-Nicolle staining

Use
  • Number of Tests possible: 230 - 300 slides.
  • Length of one Test: 3 minutes.

Principle
Gram-Nicolle staining is a differential staining based on the structure of the bacteria wall which is different whether it concerns Gram-positive bacteria or Gram-negative bacteria.

Kit components
  • Carbolic Gentian Violet: 240 ml.
  • Lugol, PVP-stabilized solution: 240 ml.
  • Fast Differentiator (Alcohol/Acetone): 240 ml.
  • Ziehl Carbolic Fuchsin 1/10: 240 ml.

Single bottles:
Code Description Volume
ZR320960-1000 Carbolic Gentian violet 1 L
ZR320960-2500 Carbolic Gentian violet 2,5 L
ZR367400-1000 Lugol, PVP-stabilized solution 1 L
ZR367400-2500 Lugol, PVP-stabilized solution 2,5 L
ZR367300-1000 Lugol solution 1 L
ZR367300-2500 Lugol solution 2,5 L
ZR361510-1000 Fast Differentiator (Alcohol/Acetone) 1 L
ZR361510-2500 Fast Differentiator (Alcohol/Acetone) 2,5 L
ZR363030-1000 Slow Differentiator (Alcohol-based) 1 L
ZR363030-2500 Slow Differentiator (Alcohol-based) 2,5 L
ZR320490-0500 Ziehl Carbolic Fuchsin 500 ml
ZR320490-1000 Ziehl Carbolic Fuchsin 1 L
ZR320490-2500 Ziehl Carbolic Fuchsin 2,5 L
ZR364540-1000 Ziehl Carbolic Fuchsin 1/10 1 L
ZR364540-2500 Ziehl Carbolic Fuchsin 1/10 2,5 L
Code
Description
Description
Volume
Price
/ Per
Package
Quantity
Total
ZR364320-0000
Kit Gram-Nicolle 4 x240ml, Ral diagnostics 
Kit Gram-Nicolle  
4 x 240 ml
40,73
/ piece
  • BOX
  • 1
  • 1

Quantity needs to be a multiple of one of the packages.

0,00
Total 0,00

These products were successfully added to your cart:

Prices are VAT excluded.

Gram-Hücker staining

Use
  • Number of Tests possible: 230 - 300 lames.
  • Length of one Test: 3 minutes.

Choosing between "R" or "L"
The "R" (fast action) kit is ideal for staining with automates and for manual and simultaneous staining of slides (series).
The "L" (slow action) kit is advised for individual staining of slides.

Principle
Gram-Hücker staining is based on the same principle as the Gram-Nicolle staining. The structure of the bacterial walls and their permeability to alcohol help differentiate the Gram-negative bacteria from the Gram-positive ones. In this technique, Carbolic Gentian Violet is replaced by Crystal Violet Oxalate (that does not require filtration) and Ziehl-Carbolic Fuchsin by Safranin.

Single bottles:
Code Description Volume
ZR361490-1000 Crystal Violet Oxalate 1 L
ZR361490-2500 Crystal Violet Oxalate 2,5 L
ZR367400-1000 Lugol, PVP-stabilized solution 1 L
ZR367400-2500 Lugol, PVP-stabilized solution 2,5 L
ZR367300-1000 Lugol solution 1 L
ZR367300-2500 Lugol solution 2,5 L
ZR361510-1000 Fast Differentiator (Alcohol/Acetone) 1 L
ZR361510-2500 Fast Differentiator (Alcohol/Acetone) 2,5 L
ZR363030-1000 Slow Differentiator (Alcohol-based) 1 L
ZR363030-2500 Slow Differentiator (Alcohol-based) 2,5 L
ZR361500-1000 Safranin 1 L
ZR361500-2500 Safranin 2,5 L
Code
Description
Description
Volume
Price
/ Per
Package
Quantity
Total
ZR361520-0000
Kit Gram-Hücker R fast action, Ral diagnostics 
Kit Gram-Hücker R, "fast action"  
4 x 240 ml
32,94
/ piece
  • BOX
  • 1
  • 1

Quantity needs to be a multiple of one of the packages.

0,00
ZR362860-0000
Kit Gram-Hücker action lente, Ral diagnostics 
Kit Gram-Hücker L, "slow action"  
4 x 240 ml
32,94
/ piece
  • BOX
  • 1
  • 1

Quantity needs to be a multiple of one of the packages.

0,00
Total 0,00

These products were successfully added to your cart:

Prices are VAT excluded.